Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice
Identifieur interne : 000B40 ( Main/Exploration ); précédent : 000B39; suivant : 000B41Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice
Auteurs : Stephanie J. Munger [États-Unis] ; Xin Geng [États-Unis] ; R. Sathish Srinivasan [États-Unis] ; Marlys H. Witte [États-Unis] ; David L. Paul [États-Unis] ; Alexander M. Simon [États-Unis]Source :
- Developmental biology [ 0012-1606 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Connexine 43 (génétique), Connexine 43 (métabolisme), Connexines (génétique), Connexines (métabolisme), Facteurs de transcription Forkhead (génétique), Facteurs de transcription Forkhead (métabolisme), Facteurs de transcription NFATC (génétique), Facteurs de transcription NFATC (métabolisme), Facteurs temps, Immunohistochimie, Phénotype, Souris de lignée C57BL, Souris knockout, Valves veineuses (croissance et développement), Valves veineuses (embryologie), Valves veineuses (métabolisme).
- MESH :
- croissance et développement : Valves veineuses.
- embryologie : Valves veineuses.
- génétique : Connexine 43, Connexines, Facteurs de transcription Forkhead, Facteurs de transcription NFATC.
- métabolisme : Connexine 43, Connexines, Facteurs de transcription Forkhead, Facteurs de transcription NFATC, Valves veineuses.
- Animaux, Facteurs temps, Immunohistochimie, Phénotype, Souris de lignée C57BL, Souris knockout.
English descriptors
- KwdEn :
- Animals, Connexin 43 (genetics), Connexin 43 (metabolism), Connexins (genetics), Connexins (metabolism), Forkhead Transcription Factors (genetics), Forkhead Transcription Factors (metabolism), Immunohistochemistry, Mice, Inbred C57BL, Mice, Knockout, NFATC Transcription Factors (genetics), NFATC Transcription Factors (metabolism), Phenotype, Time Factors, Venous Valves (embryology), Venous Valves (growth & development), Venous Valves (metabolism).
- MESH :
- chemical , genetics : Connexin 43, Connexins, Forkhead Transcription Factors, NFATC Transcription Factors.
- chemical , metabolism : Connexin 43, Connexins, Forkhead Transcription Factors, NFATC Transcription Factors.
- embryology : Venous Valves.
- growth & development : Venous Valves.
- metabolism : Venous Valves.
- Animals, Immunohistochemistry, Mice, Inbred C57BL, Mice, Knockout, Phenotype, Time Factors.
Abstract
Venous valves (VVs) are critical for unidirectional blood flow from superficial and deep veins towards the heart. Congenital valve aplasia or agenesis may, in some cases, be a direct cause of vascular disease, motivating an understanding of the molecular mechanisms underlying the development and maintenance of VVs. Three gap junction proteins (Connexins), Cx37, Cx43, and Cx47, are specifically expressed at VVs in a highly polarized fashion. VVs are absent from adult mice lacking Cx37; however it is not known if Cx37 is required for the initial formation of valves. In addition, the requirement of Cx43 and Cx47 for VV development has not been studied. Here, we provide a detailed description of Cx37, Cx43, and Cx47 expression during mouse vein development and show by gene knockout that each Cx is necessary for normal valve development. The valve phenotypes in the knockout lines exhibit Cx-specific differences, however, including whether peripheral or central VVs are affected by gene inactivation. In addition, we show that a
Url:
DOI: 10.1016/j.ydbio.2016.02.033
PubMed: 26953188
PubMed Central: 4826804
Affiliations:
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Sathish" last="Srinivasan">R. Sathish Srinivasan</name><affiliation wicri:level="1"><nlm:aff id="A2">Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK</wicri:regionArea><wicri:noRegion>OK</wicri:noRegion></affiliation></author><author><name sortKey="Witte, Marlys H" sort="Witte, Marlys H" uniqKey="Witte M" first="Marlys H." last="Witte">Marlys H. 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Simon</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Physiology, University of Arizona, PO Box 245051, Tucson, AZ 85724, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physiology, University of Arizona, PO Box 245051, Tucson, AZ 85724</wicri:regionArea><wicri:noRegion>AZ 85724</wicri:noRegion></affiliation></author></analytic><series><title level="j">Developmental biology</title><idno type="ISSN">0012-1606</idno><idno type="eISSN">1095-564X</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Connexin 43 (genetics)</term><term>Connexin 43 (metabolism)</term><term>Connexins (genetics)</term><term>Connexins (metabolism)</term><term>Forkhead Transcription Factors (genetics)</term><term>Forkhead Transcription Factors (metabolism)</term><term>Immunohistochemistry</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>NFATC Transcription Factors (genetics)</term><term>NFATC Transcription Factors (metabolism)</term><term>Phenotype</term><term>Time Factors</term><term>Venous Valves (embryology)</term><term>Venous Valves (growth & development)</term><term>Venous Valves (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Connexine 43 (génétique)</term><term>Connexine 43 (métabolisme)</term><term>Connexines (génétique)</term><term>Connexines (métabolisme)</term><term>Facteurs de transcription Forkhead (génétique)</term><term>Facteurs de transcription Forkhead (métabolisme)</term><term>Facteurs de transcription NFATC (génétique)</term><term>Facteurs de transcription NFATC (métabolisme)</term><term>Facteurs temps</term><term>Immunohistochimie</term><term>Phénotype</term><term>Souris de lignée C57BL</term><term>Souris knockout</term><term>Valves veineuses (croissance et développement)</term><term>Valves veineuses (embryologie)</term><term>Valves veineuses (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Connexin 43</term><term>Connexins</term><term>Forkhead Transcription Factors</term><term>NFATC Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Connexin 43</term><term>Connexins</term><term>Forkhead Transcription Factors</term><term>NFATC Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Valves veineuses</term></keywords><keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Valves veineuses</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Venous Valves</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Venous Valves</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Connexine 43</term><term>Connexines</term><term>Facteurs de transcription Forkhead</term><term>Facteurs de transcription NFATC</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Venous Valves</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Connexine 43</term><term>Connexines</term><term>Facteurs de transcription Forkhead</term><term>Facteurs de transcription NFATC</term><term>Valves veineuses</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Immunohistochemistry</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>Phenotype</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Facteurs temps</term><term>Immunohistochimie</term><term>Phénotype</term><term>Souris de lignée C57BL</term><term>Souris knockout</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Venous valves (VVs) are critical for unidirectional blood flow from superficial and deep veins towards the heart. Congenital valve aplasia or agenesis may, in some cases, be a direct cause of vascular disease, motivating an understanding of the molecular mechanisms underlying the development and maintenance of VVs. Three gap junction proteins (Connexins), Cx37, Cx43, and Cx47, are specifically expressed at VVs in a highly polarized fashion. VVs are absent from adult mice lacking Cx37; however it is not known if Cx37 is required for the initial formation of valves. In addition, the requirement of Cx43 and Cx47 for VV development has not been studied. Here, we provide a detailed description of Cx37, Cx43, and Cx47 expression during mouse vein development and show by gene knockout that each Cx is necessary for normal valve development. The valve phenotypes in the knockout lines exhibit Cx-specific differences, however, including whether peripheral or central VVs are affected by gene inactivation. In addition, we show that a <italic>Cx47</italic> null mutation impairs peripheral VV development but does not affect lymphatic valve formation, a finding of significance for understanding how some <italic>CX47</italic> mutations cause inherited lymphedema in humans. Finally, we demonstrate a striking segregation of Foxc2 and NFATc1 transcription factor expression between the downstream and upstream faces, respectively, of developing VV leaflets and show that this segregation is closely associated with the highly polarized expression of Cx37, Cx43, and Cx47. The partition of Foxc2 and NFATc1 expression at VV leaflets makes it unlikely that these factors directly cooperate during the leaflet elongation stage of VV development.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Munger, Stephanie J" sort="Munger, Stephanie J" uniqKey="Munger S" first="Stephanie J." last="Munger">Stephanie J. Munger</name></noRegion><name sortKey="Geng, Xin" sort="Geng, Xin" uniqKey="Geng X" first="Xin" last="Geng">Xin Geng</name><name sortKey="Paul, David L" sort="Paul, David L" uniqKey="Paul D" first="David L." last="Paul">David L. Paul</name><name sortKey="Simon, Alexander M" sort="Simon, Alexander M" uniqKey="Simon A" first="Alexander M." last="Simon">Alexander M. Simon</name><name sortKey="Srinivasan, R Sathish" sort="Srinivasan, R Sathish" uniqKey="Srinivasan R" first="R. Sathish" last="Srinivasan">R. Sathish Srinivasan</name><name sortKey="Witte, Marlys H" sort="Witte, Marlys H" uniqKey="Witte M" first="Marlys H." last="Witte">Marlys H. Witte</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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